I didn't think about it, but I have made margaritas from juice I've just squeezed, and squeezed beforehand and the ones that had been sitting may have been better. Key limes are better, but you get a little less juice. Finally, real men get an automated citrus juicer for the eventualities of parties, throw 30 limes in the hopper get unlimited juice, best $1000 I've ever spent.