What did you stuff the cabinet with, and is it a down-firing configuration? If it is down-firing, is the stuffing able to fall onto the back of the cone? How did you secure the brace?
Remove the woofer and use your fist to pound on the cabinet, listening for vibrations. Make sure it's not something nearby that's vibrating- I'm re-loading some speaker cabinets for a bar/restaurant and when I fired up the one I had all kinds of things making noise on my work bench.
If you have or will download Audacity (or something like it), it has a waveform generator and you can select the frequency to select it, instead of doing a sweep and finding a range. The thing to remember is that you don't want to do this at a high power level.