A few tips to expand on this... Screws are the weak point in a sub box. They help yes, but in moderation. I would saw less is more, many guys just use brads to hold together as the glue sets, for aplications with low watts like yours.
Also use WOOD glue. MDF is wood dust and wood glue, using wood glue will make it more like one solid peice of board, which is the ultimate goal.
You also asked about Glassing... well here is my opinion, your set up isn't going to be flexing the box tooo much and certianly won't be causing that much pressure. Something more important is glue glue glue and more glue. especialy if you used a circle saw and not a table saw for the cuts.
If you do a flared square(aka flared slot port) then you may want to toss down some kitty hair and glass it up, otherwise nothing else is needed using 3/4 MDF board and plenty of glue with air tight seals you will be more than fine sir. If you were going to go up into the 1,000 pluss watt range etc, then it may be worth it, but even then doubling up on the MDF would be a better option IMHO.