Look up a video, itl make more sense than me lol.
Essentially your going to turn the gain all the way down. Next your going to turn the radio up to a volume a little higher than your preferred listening level, with volume at that level you will turn the gain up slowly on the amp, when the sub 'bumps, rattles, distorts' (you'll easily tell) turn the gain back down till it stops.
Some people will set the volume too high while doing this, in my experience it's a bad thing for normal listening levels.