great let us know, you want to isolate the problem with the process of elimination method, but swapping speakers and cables from L to R you eliminate them as a source of the problem, then eliminate that amplifier by swapping channels, ect
I would also try unplugging and replugging all the tubes in the same spot, sonce you know which is the problem your tube amp or tube pre, then you can swap the tubes around to see which if any are the issue, I hope its not a cap or something internal... good luck..