I have measured(several measurements in each state to rule out measurement anomalies) this previously. I used 3 way main speakers, with a lower tuning of about 27 Hz, ported. I placed a medium sized unpowered subwoofer in the room, it was tuned to about 29 Hz, ported, and 10" diameter cone with 3" diameter port. No substantial difference was measured in the lower frequency band, but curiously, a null that was centered around 160 Hz was substantially alleviated with the subwoofer in the room. I wondered if it was just the physical placement of the subwoofer as an obstruction device, so I moved it to new location(from left rear corner to near front left corner of room) and the effect still existed. Is the difference audible? Perhaps in a very sensitive comparison such as ABX. But casually, I did not notice anything that I could not be sure was not imagined. I would have to record music with content around the band of the null with the subwoofer in and out of the room, time sync both recordings, then compare in a proper blind testing software system.
-Chris