You don't really hear 16Hz, but you do FEEL it. Not everyone hears the same, but the average person should be able to hear 20Hz. In my setup with the PB-10, you could hear 18Hz still, and 16Hz was pretty much just vibration, but that tactile rumble does add to the experience for sure. Below about 25Hz, you can't tell where the bass is coming from because it is
everywhere, activating the air in the room because the wavelenghts are longer than most rooms. Real world, there is a definite, audible difference between 36Hz and 20Hz.
To achieve very long lengths for ports, you would use multiple ports sized appropriately to give the same effect as a single long port.
Without the tools to measure what you've built, I think you're going to have a difficult time designing this sub from scratch. I don't know if I recommended it to you or not, but check out
these from GR Research. He'll provide you with plans for the correctly sized box; add an amp and you're good to go. No guesswork.